The charge is under article 2.13, which relates to the personal abuse of a player, player support personnel, umpire or match referee during an global match.
The on-pitch microphones did not pick up what Gabriel said, but umpires Rod Tucker and Kumar Dharmasena have charged the bowler with breaking the International Cricket Council's (ICC) code of conduct, according to Reuters.
Joe Root has suggested that West Indies bowler Shannon Gabriel made a regrettable chirp involving homosexuality during their on-field tussle in the third Test. Footage only showed root responding to the player, saying "There is nothing wrong with being gay".
"Gabriel already had five demerit points against his name for two earlier incidents - three demerit points during the Jamaica Test against Pakistan in April 2017 and two demerit points in the Chittagong Test against Bangladesh last November", the ICC said in a statement.
"The charge, which was laid by match umpires, will now be dealt with by Match Referee Jeff Crowe".
"West Indies have played some fantastic cricket, they are a good bunch of guys and it would be a shame if it tarnishes it".
Root would not say after stumps what had been said.More news: Retail inflation cools further to 2.05% in January on easing food prices
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"That battle was a good contest, he's had a wonderful series and he should be proud. I don't want anything said in the middle to ruin what's been a good test series for him and his team".
"The more players, fans, clubs and organisations that stand up for equality in sport, the sooner we kick discrimination out and make sport everyone's game".
Hussain tweeted: "I don't know who said what to whom... but boy do I applaud Joe Root's reaction here".
"Language is really influential and it's great Joe Root stepped up to challenge abusive comments".
UK Sport Minister Mims Davies was among others who praised Root for his stance. The pressure was on and England's captain Joe Root was looking at me intensely as I prepared to bowl, which may have been the usual psychological strategy with which all Test cricketers are familiar.
On Wednesday, West Indies coach Richard Pybus told the BBC he had yet to receive a report of the incident but that action would be taken if anything unsavory had happened.