However, Bono never returned to stage with his bandmates - Adam Clayton, Larry Mullen and The Edge - releasing a message shortly afterwards, revealing they were seeking "medical advice".
The Irish quartet kicked off their eXPERIENCE & iNNOCENCE Tour in Berlin's Mercedes-Benz Arena last night - but the band's second show in the same city came to an abrupt end as the frontman lost his voice onstage. Something has happened and I think we can't go on.
The band said that they don't know what happened, but they're taking medical advice on the issue.
For the second time since 1989, U2 has had to cancel a concert partway through after Bono's voice failed, reports E! "As always, we appreciate our audience's understanding and all our fans" support in Berlin and those who travelled from afar.
"I've seen a great doctor", the singer said in a statement, adding that he was "happy and relieved that anything serious has been ruled out".
News that the singer was struck mute on stage in Berlin right at the start of his tour has therefore caused much amusement, with Labour Leave activist and trade union leader Paul Embery quipping: "There is a God".More news: Serena Williams Hails Colin Kaepernick's Dedication To 'Greater Good'
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"My relief is tempered by the knowledge that the Berlin audience were so inconvenienced", he continued.
"We can't wait to get back there on November 13th".
In a video posted by a fan of the Saturday night concert, Bono appeared to be coughing and told fans he was "so sorry".
So far, the band is still slated to continue their tour with their next show in Cologne on September 4th.
An official announcement is expected to be made on U2.com in the coming days regarding any possible rescheduling.