Iranian President Hassan Rouhani on Sunday warned the US against taking aggressive action that could lead to war with Iran.
Tensions between the USA and Iran have been heightened for years, dating back well before the Trump administration.
"As I have previously said, we can not trust in the words of the United States and even in their signature, so negotiations with the United States are useless", Khamenei told a gathering of Iranian diplomats in Tehran.
Europe is opposed to the move and has vowed to find ways of maintaining its trade ties with Iran, which under the deal curbed its nuclear program in exchange for a lifting of sanctions. The same threat was earlier voiced by President Hassan Rouhani.
"You are not in a position to incite the Iranian nation against Iran's security and interests", Rouhani added, Reuters reported.
Mr. Netanyahu, a steadfast opponent of the nuclear deal since it was signed in 2015, in response accused Iran of "trying to conquer" the Middle East with its military presence in Syria.More news: 4Burberry burns millions in unsold products to protect brand
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Iran's Supreme Leader Ayatollah Ali Khamenei has supported a suggestion by President Hassan Rouhani who hinted earlier this month that Tehran may block regional oil exports if its own sales are stopped following the US' withdrawal from a 2015 nuclear deal with world powers.
Rouhani repeated his warning that Iran could shut down the strategic Strait of Hormuz, a vital shipping lane for worldwide oil supplies.
Iranian officials have in the past threatened to block the Strait of Hormuz in retaliation for any hostile USA action.
"I have previously pointed out that Americans' words or even signatures can not be relied on; thus negotiations with the USA are useless".
"The United States seeks to restore its status in Iran as they enjoyed before the Islamic Revolution (in 1979), and they will not accept less than that", Khamenei said in a meeting with the Iranian Foreign Ministry staff in the capital Tehran.
But it has somewhat eased its stance since, saying that it may grant sanction waivers to some allies that are particularly reliant on Iranian supplies.